Gambar:Summary of SAGE.svg
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Ringkesna
| Pedaran |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| Titimangsa | |
| Sumber | Karya sorangan |
| Pangarang | Thomas Shafee |
| Versi lainnya |
Also in series:
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Lisénsi
Saya, pemilik hak cipta dari karya ini, dengan ini menerbitkan berkas ini di bawah ketentuan berikut:
Berkas ini dilisensikan di bawah lisensi Creative Commons Atribusi 4.0 Internasional.
- Anjeun bébas:
- pikeun babagi – pikeun nyalin, nyebarkeun, sarta nyalabarkeun karyana
- pikeun nyampurkeun – pikeun ngarobah karyana
- Dumasar kana kaayaan di handap:
- atribusi – Anjeun kudu ngatribusi karya ku cara nu geus ditangtukeun ku nu nyieun atawa nu méré lisénsi (tapi lain ku jalan nu sigana téh maksa pikeun Anjeun maké éta karya).
- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
Jujutan berkas
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| Titimangsa | Miniatur | Ukuran | Pamaké | Kamandang | |
|---|---|---|---|---|---|
| kiwari | 13 Pébruari 2017 12.21 | | 938 × 756 (180 KB) | Evolution and evolvability | text as paths to avoid artefacts |
| 13 Pébruari 2017 10.48 |  | 938 × 756 (104 KB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| 7 Oktober 2016 05.27 |  | 938 × 756 (104 KB) | Evolution and evolvability | ce | |
| 7 Oktober 2016 05.23 |  | 938 × 756 (102 KB) | Evolution and evolvability | ce | |
| 7 Oktober 2016 05.16 |  | 938 × 756 (104 KB) | Evolution and evolvability | digitag-->tag | |
| 5 Oktober 2016 05.08 |  | 1.020 × 821 (106 KB) | Evolution and evolvability | User created page with UploadWizard |
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